Coding

Part:BBa_K5226011:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-29)


Vib-lpd


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 397
    Illegal SpeI site found at 478
    Illegal PstI site found at 191
    Illegal PstI site found at 668
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 397
    Illegal NheI site found at 942
    Illegal NheI site found at 1060
    Illegal SpeI site found at 478
    Illegal PstI site found at 191
    Illegal PstI site found at 668
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 397
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 397
    Illegal SpeI site found at 478
    Illegal PstI site found at 191
    Illegal PstI site found at 668
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 397
    Illegal SpeI site found at 478
    Illegal PstI site found at 191
    Illegal PstI site found at 668
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Sequence source is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/CP009978.1?from=1576325&to=1577719）

References